Ammonia Oxidizing Bacteria in Industrial Waste Water

Denaturing Gradient Gel Electrophoresis is a influential and feasible device for sequence examination of the variety of complicted natural microbial populations. For PCR amplification of 16S rDNA products and specific primers were used and analyzed by denaturing gel electrophoresis in gradient. Probes specific for the cluster Genusand were designed to bind to sequences within the region amplified by these primers. Specific sequence of all b-subgroup of the oxidizing ammonia could not be identified, but probes specific for Nitrosomonas Nitrosospira and were designed. The presence of the Nitrosomonas cluster and Nitrosospira clusters were confirmed by denaturing electrophoresis banding patterns on gradient gel, but the results were ambiguous due to the overlapping of banding patterns. Compared to the internal control sensor signals 3 sensors Nitrosospira group decreased significantly with decreasing pH in the range of 06/05 to 03/08, while Division 2 Nitrosospira hybridization signal increases with acidity of the ground. At pH 5.4, cluster munitions Nitrosospira 4 signals were more, the lower upper and lower values, while the Nitrosomonas group of signals does not vary significantly with pH. Different sets of primers for PCR amplification of the 16S rDNA sequences of the soil used in both studies and similar results demonstrate that PCR is unlikely to be an important factor. These results reveal the value of DGGE and testing for rapid analysis of b-subgroup protéobactériens communities, "oxidizing ammonia, pH differences indicates related capitals Nitrosospira populations, and shows that the cluster may have Nitrosospira importance for the ammonia-oxidant in acidic soils.